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SIBLE, G. V.
- Estimation of Genetic Diversity in Nutmeg (Myristica fragrans Houtt.) Selections Using RAPD Markers
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Affiliations
1 Regional Agricultural Research Station (KAU), Kottayam (Kerala), IN
1 Regional Agricultural Research Station (KAU), Kottayam (Kerala), IN
Source
International Journal of Plant Sciences, Vol 12, No 2 (2017), Pagination: 102-107Abstract
Nutmeg (Myristica fragrans Houtt.) is an evergreen tree and popular spice. In the present study, Random Amplified Polymorphic DNA (RAPD) analysis was performed to assess the genetic diversity among the 19 superior accessions of nutmeg collected from different geographic locations and maintained in the germplasm collections of Regional Agricultural Research Station, Kumarakom, Kerala. This included one released variety IISR Viswasree. Out of 28 RAPD primers tested, 20 were amplified. Out of the 109 loci amplified, 82 were polymorphic with an average polymorphism rate of 72.74 per cent. The number of bands for each primer ranged from 2 to 8. The markers which produced maximum number of polymorphic bands were BB-18 and PO-5. PIC value of the markers ranging from 0.09 (OPA 11) to 0.48 (W-15) with an average of 0.31. The marker index (MI) varied between 0.09 and 2.08 with an average of 1.25. Jaccard’s similarity co-efficient of the genotypes ranged between 0.34-0.93. Dendrogram constructed based on UPGMA analysis grouped the 19 selected genotypes into two major clusters. The knowledge on genetic diversity of nutmeg can be used for further breeding programmes for getting higher nut and mace yield.Keywords
Nutmeg, Genetic Diversity, RAPD, Dendrogram.
References
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- Antagonistic Efficacy of Trichoderma Isolates Against Soil-Borne Plant Pathogens, Pythium Aphanidermatum and Rhizoctonia Solani
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Authors
Affiliations
1 Department of Plant Pathology, College of Agriculture, Vellayani, Thiruvanathapuarm – 695522, Kerala, IN
2 Regional Agricultural Research Station, Kumarakom, Kottayam – 686563, Kerala, IN
3 Department of Plant Pathology, College of Agriculture, Padannakkad, Kasaragod – 671 314, Kerala, IN
1 Department of Plant Pathology, College of Agriculture, Vellayani, Thiruvanathapuarm – 695522, Kerala, IN
2 Regional Agricultural Research Station, Kumarakom, Kottayam – 686563, Kerala, IN
3 Department of Plant Pathology, College of Agriculture, Padannakkad, Kasaragod – 671 314, Kerala, IN
Source
Journal of Biological Control, Vol 35, No 2 (2021), Pagination: 48-56Abstract
Trichoderma spp. are long been recognized as efficient fungal biocontrol agents for the control of plant disease and for their ability to increase plant growth and development. Management of soil borne diseases has become very much important since it causes high crop yield losses. The present study was carried out to isolate Trichoderma spp. from soil samples collected from different locations of Kerala and to test their in vitro efficacy against soil borne pathogens viz., Pythium aphanidermatum and Rhizoctonia solani. The Trichoderma spp. was isolated on Trichoderma Selective Medium (TSM) and observed that the isolates differed in radial growth and colony characters such as colony colour, texture and sporulation. In vitro studies revealed the potential of Trichoderma isolates against soil borne pathogens. Isolates TRPN3 and TRPN7 exhibited no sporulation and white mycelial colour. Isolates which completed their growth at four days after inoculation include TRKR1, TRPN3, TRPN7, TRPN10 and TRPN18. Biocontrol activities against different pathogens resulted in inhibition of pathogens. Maximum inhibition percentage was observed by the isolates TRPN7, TRPN15 and TRKR2 against both the pathogens. The maximum inhibition exhibited against both the pathogens is due to the antagonistic property displayed by the isolates.Keywords
Antagonistic Activity, Inhibition, Soil Borne Pathogens, Trichoderma Spp.Full Text
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- Characterization of novel strains of Trichoderma spp. and their utilization in management of damping off disease in tomato
Abstract Views :100 |
PDF Views:83
Authors
ATHIRA NAIR
1,
G. V. SIBLE
2,
ANIT CYRIAC
1,
SUSHA S. THARA
1,
JOY MICHAL JOHNSON
3,
N. S. RADHIKA
4,
K. B. SONI
5
Affiliations
1 Department of Plant Pathology,, IN
2 Regional Agricultural Research Station, Kumarakom, Kottayam - 686563, Kerala, India., IN
3 arming Systems Research Station, Sadanandapuram, Kollam - 691531, Kerala, India ., IN
4 Department of Plant Pathology, College of Agriculture, Padannakkad, Kasaragod - 671314, Kerala, India ., IN
5 Department of Plant Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram - 695522, Kerala, India ., IN
1 Department of Plant Pathology,, IN
2 Regional Agricultural Research Station, Kumarakom, Kottayam - 686563, Kerala, India., IN
3 arming Systems Research Station, Sadanandapuram, Kollam - 691531, Kerala, India ., IN
4 Department of Plant Pathology, College of Agriculture, Padannakkad, Kasaragod - 671314, Kerala, India ., IN
5 Department of Plant Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram - 695522, Kerala, India ., IN
Source
Journal of Biological Control, Vol 36, No 1 (2022), Pagination: 31 - 46Abstract
Chemical fungicides used in plant disease management may have deteriorative effects on humans, animals, and the environment. The use of native strains of Trichoderma spp. against plant diseases may help to reduce the dependence on chemical fungicides. In this study, eleven novel isolates of Trichoderma spp. from virgin forest soils of different agro-climatic zones of Kerala were characterized and evaluated for their efficacy against damping off disease of tomato caused by Pythium aphanidermatum under in vitro and in vivo; and also, against wilt pathogen, Fusarium oxysporum under in vitro conditions. Dual culture assay showed that all the Trichoderma isolates were found to inhibit the growth of P. aphanidermatum and F. oxysporum under in vitro conditions with multiple modes of action. The mycelial colour, texture, and conidial characters varied among all the isolates. The volatile metabolites by isolates of Trichoderma spp. also showed in vitro inhibition of the pathogens. Seed treatment (20 g kg-1) and potting medium addition @ 2 % (w/w) of isolates TRMW-2, TRKR-2, TRPN-3, TRPN-11 and TRPN-17 could effectively reduce pre- and post-emergence damping off of tomato. Among them, isolates TRMW-2, TRKR-2, and TRPN-11 were the most effective ones in reducing pre- and post-emergence damping off to about 72 and 90 percent respectively. Molecular identification of the isolates of Trichoderma spp. using ITS universal primers revealed similarity with certain reference strains of the NCBI Genbank database.Keywords
Biocontrol, damping off, Fusarium oxysporum, Pythium aphanidermatum, Trichoderma, wiltFull Text
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